Monday, June 30, 2014

Almost My Entire Week!

The week has gone by so quickly! Here's a summary of almost all of last week:

Tuesday

In the morning, Jorge helped Flavio and me clean off the potato plants we had discovered on Monday.  After washing them, we used them as practice for the large number of frozen roots we had to analyze.  



Jorge helping prepare the plants

First we stretched the roots out and measured to the longest point before counting the number of roots.  Next, we laid the roots out on an office scanner to scan and input into a computer program.  After seeing the first couple scans, I suggested that we lay something black over the top for better contrast, so Flavio and I scoured the surrounding buildings until we found a black, felt-like cloth that worked perfectly.  


Once we had several scans to work with, Flavio and I began to search the internet for free, high-quality software that would analyze the root scans.  It was actually terribly difficult.  So I left work on Tuesday slightly disappointed that we had come up empty on our search. 


Wednesday

I started my day at CIP with a trip to the fields with Ximena and Elisa.  They explained that the field workers were planting about one thousand little diploid potato plants for field testing. They were performing the whole task by hand – spreading the fertilizer, measuring and arranging the plants according to the field design, and eventually planting each one.  It was incredible to see!

Each of the blue trays had about twenty plants for the row section

Next, Ximena and Elisa took me to a special greenhouse where they are testing potato varieties for resistance to a certain disease.  Apparently, this disease is unique because they cannot ‘mechanically’ (as they said) transmit it to potatoes, so they devised a clever system for transmission.  

First, they grow a plant that attracts slews of aphids.  Then they transfer those aphids to potato plants that are susceptible to the disease and are confirmed carriers.  The aphids feed on the infected potato leaves, and then carry the disease.  

The plants that attracted aphids
They were covered in aphids

Elisa explained that our job was to pluck off handfuls of the infected potato stems and then “shake, shake, shake” them into the bags of potatoes that were being tested.  After vigorously shaking the leaves, you put any leaf that had aphid stragglers into the bags.  It was a very effective method, but it felt a bit odd, and Elisa jokingly told me that we looked like “brujas” or witches!

My clump of infected leaves


Afterwards, I went to the aeroponics greenhouse because David and Luciano were going to show me the unique way they planted potatoes.  First they selected nine fairly uniform plants from each genotype that they were testing, and then cleaned the roots before removing the seed potato tuber and the lower leaves.  Then Luciano explained how to fit the roots into a drinking straw-like piece of plastic that allowed them to carefully insert the plants into the aeroponic chambers. 



Luciano let me try my hand at it

Success!

After preparing the plants, Luciano planted them individually by punching a hole through the plastic and Styrofoam-like top of the chamber, and then inserting the plant through the hole and pulling it through and the straw-like holder off.  The roots freely extend into the open chamber below, and the side panels can be removed to check their growth progression. It was quite fascinating!

All the planting materials

That afternoon, I returned to do more root scans.  I was thrilled to discover that Flavio had found a program that met the qualifications, and we could begin on the real samples.  The process was mostly the same, but we also had to thaw the roots in a water bath and then carefully tease them apart for a more accurate scan. We only finished four, but it was progress!

Liliam was planning to stay late at work, so I decided to walk home.  As I was mapping out my route at work, I just happened to find that there are in fact Starbucks stores in Peru, and one was on the way home from work! I gave in to the temptation and it was worth it. Not only could I enjoy a taste of home, but I could do so in an incredibly cool two-story Starbucks building!


Thursday

When I got to my desk at CIP on Thursday morning, I noticed that Dr. Khan’s door was open. He was back! I was excited to finally meet my mentor as he has been gone on business trips for the entirety of my internship so far. We were able to meet and talk briefly, and Dr. Khan was very nice and even brought back chocolates from Switzerland to share with the group!

In the morning, Flavio and I went out to the greenhouse to water the pots.  One of the computer programs calculated that each plant needs about 375 mL of water.  So Flavio and I measured out and poured that amount on all 180 plants. It took a while.

Afterwards, Flavio and I continued with the root analysis, and we’ve got it down to about 20 minutes per sample. We quickly ate lunch because I went to a tour of the CIP Genebank at noon.  The tour was fascinating! 

Some of the potatoes, the one in the top left corner is
believed to be the first variety of domesticated potatoes


They came in all different shapes, sizes, and colors

Dra. Ana Panta showed me several different types of potatoes and tubers and their uses and benefits to the indigenous farmers.  She also let me peek into the two different storage rooms where they keep about ten thousand different varieties from all over the world.  Also, Dra. Panta explained that there are different methods that they utilize to maintain this biodiversity – they keep planting them in different CIP research stations across Peru, they store plantlets in either the cold or warm storage rooms, or they cryogenically freeze them and store them in liquid nitrogen. 

Cold storage
Warm storage
After the tour, I went back to my desk for a while to do some reading; but I may or may not have kept checking the USA vs. Germany World Cup game (we lost, but continue on to the elimination rounds, go USA!!).  Then Flavio and I went back to the root analysis for the rest of the afternoon.


Friday

On Friday morning, Flavio and I went out to the greenhouse to check on the plants, and we found some sprouts emerging! Then we continued the root analysis for a couple hours before we went to the Gathering under the Tree. 


The Gathering under the Tree is a meeting that happens every month (I think) where the CIP employees come together in the central garden for some refreshments and listen to announcements and short speeches.  The drink that was offered was called “coco de maca” and it was similar to hot cocoa but they had added maca, which is an Andean tuber.  It was delicious!

Also, Dr. Khan introduced me to Dave Ellis who is in charge of the CIP Genebank and we talked for a short while.  Then, I had the great honor of meeting Barbara Wells, the Director General of CIP!  She came over and introduced herself to me, and we had the chance to speak for a few minutes before the ceremony started.  It was such an incredible opportunity!

For the rest of the day, Flavio and I worked on the root analysis, with a break for lunch.  On Friday, there were no World Cup games to play during lunch, so when I walked into the cafeteria I heard Frank Sinatra playing on the sound system.  It was a nice, but very unexpected change of pace! Also, I loved these little chicken wontons that were served.


After work, I decided to walk home because it was a beautiful, sunny day.  On the way, I found that three-story grocery store that Liliam had taken me to earlier and decided to explore it a bit more. I’m also running out of the snacks that I brought with me, so I thought I’d try a few new ones. 



Looking down from the second floor

First I got an Inca Kola, which is a widely popular Peruvian soda that I heard tasted like bubble gum, so I wanted to try it for myself.  I also got a Coke, some purple corn tortilla chips (purple corn is native to Peru), some Lay’s chips that were part of their country themed “Copa de los Sabores” flavors and I picked the French cheese flavored ones, as well as some sort of chocolate wafer cookies called Cua Cua.  Over the past few days, I’ve had the chance to try all of the different things that I purchased and have concluded the following: Inca Kola does actually taste like a fizzy, lemony bubble gum and I’m still not sure what I think of that, Coke is always a favorite, the purple corn tortilla chips tasted similar to blue corn tortilla chips but they also had sesame seeds that added a nice flavor, the Lay’s chips tasted similar to the sour cream and onion flavor but with a bit less onion and more cheese, and I really liked the cookies.


Once I got home, Liliam and Daniel invited me to a 9th birthday party for a family member. Alejandro had a Dragon Ball Z-themed party and it was actually kind of crazy.  There were dozens of eight to ten year olds, blaring music, disco lights, a clown, and tons of food! The clown was more like a comedic entertainer who had fun games and activities for the kids, and I thought he was actually pretty funny even though I couldn’t understand about half of what he was saying.  


After the clown act, some of the family members carried around trays of different foods to the adults sitting at the perimeter of the room.  The food included hot dogs (that had a much different texture and taste than American ones), sausages, cheese, animal crackers, Jello cups, taquito-style sausage sticks, and these delicious balls called causa.  I found out that they consist of mashed yellow potatoes, a Peruvian yellow pepper, lime juice, and salt. They were amazing, and so fresh tasting!


Saturday

I began Saturday with a small breakfast before tidying my room up and having some time to relax.  I ate lunch with Liliam and Daniel and they served me this Peruvian fruit that they call tuna. It's a cactus fruit and the inside is bright red and has a very similar taste and texture as watermelon.  


Then I set off with Myra (not sure on the spelling), who is a relative of Liliam’s.  Myra is in her twenties and is just finishing studying law at her university.  Myra and I took a taxi into the center of Lima and started at a long boulevard that was lined with shops and packed with people.  



Afterwards, she took me to the San Martín square and it was surrounded by beautiful, old colonial-era buildings.  Then Myra showed me this incredibly old and ornate church that was filled with shrines and statues and beautiful decorations. 
With the statue of Jose de San Martin


Later, we toured the Gastronomy Museum that depicted dozens of crops, foods, and cooking tools and methods throughout the centuries in Peru.  After my CIP Genebank tour, it was especially interesting to see all of the different varieties and uses for potatoes throughout Peruvian history.

Little jugs that the indigenous peoples used for drinking

A representation of an altarpiece in Peru where they
depict the Last Supper as a traditional meal of cuy (guinea pig)
After that museum, Myra and I went to a gallery that was showcasing work of a local photographer that captured street views in different districts of Lima. The photos were all colorful, but while some were filled with happiness, others depicted heart-wrenching poverty and desperation. It was all quite moving.


Next, we visited the gorgeous old building where the mayor of Lima works.  We were able to walk around the atrium and the stairs, but otherwise the building was closed for the weekend.


At about 5:00, Myra and I bought bus tickets for a short ride up to an outlook in San Cristobal (one of the districts in Lima).  The trip wove through some of the poorer parts of town, and it was unbelievable to see all of the colorful houses crammed together with narrow roads woven throughout.  


Our trip took us up an enormous hill/small mountain that had incredible views of Lima stretching out in all directions.  As the sun set, it was even more beautiful to see the webs of twinkling lights crisscrossing the land. 
Myra and I at the top
 

Also, the summit was topped with this massive cross and little shrines.  Myra explained that it was a tradition to buy a colored candle based on what you wanted (green for money, red for love, etc.) and to light it and place it in a holder at the base of the cross.  So I purchased a pink candle signifying friendship and followed the tradition.  Shortly thereafter, we returned to the bus and made our way back to Lima, and all along the way I could see the cross lit up like a beacon in the night.



Once we made it back to central Lima, Myra took me to a shopping area that was like a cross between a small mall and a marketplace.  It was filled with little stalls absolutely bursting with colors and products.  It was a bit overwhelming to look through all of the stuff, but finally I settled on some pencil bags with colorful stitching depicting local animals and patterns, and some hand-painted gift bags with portrayals of native Peruvian women.

Then, Myra and I made our way back to La Molina (the district that I live in) and ate dinner at a restaurant called Mi Tradición.  Myra recommended that I try chicha morada which is a sweet drink made from purple Peruvian corn, and it was great.  We then shared a traditional dish of anticuchos, which are seasoned pieces of beef (that are actually beef heart, as I found out later) that are put on skewers and served with corn and potatoes.  


Then we finished off the meal with picarones which are like doughnuts served with a light sauce that tasted like a cross between maple syrup and molasses.  The entire meal was incredible, and it was fun to try some more traditional Peruvian dishes!



Sunday

I had a nice relaxing day today after my adventures on Saturday.  This morning, I slept in until about 9:00 before having breakfast.  Afterwards, I started writing this (incredibly long, I’m sorry) blog post.  We had a late lunch of beef, rice, corn, and tomatoes before I got ready to go to the mall with Liliam. I watched some of the Costa Rica vs. Greece World Cup game with Daniel before heading out. 

Liliam and I went to this absolutely enormous mall in a neighboring district. The mall had many similarities to those in the United States in terms of stores and atmosphere, but it was much more open than I expected.  There weren’t really any doors to enter the mall and there were several open-air plazas throughout.  I initially, as an Iowan, thought that this would be horrible in the wintertime because what would they do when it snowed, before remembering that I am in an incredibly temperate area where it is actually wintertime J



We visited some department stores and clothing stores where Liliam bought a couple of things.  Then we went to the food court area.  I was surprised because the majority of the food stores were American chain restaurants like Starbucks, KFC, Subway, Pizza Hut, Dunkin Doughnuts, Chili's, and TGI Friday's. Also, the food court was absolutely packed with people because they were showing the World Cup game on an enormous screen.  

The food court with the World Cup showing

Liliam bought me a personal pizza from Pizza Hut for dinner.  She ordered the American style, which ironically was Canadian bacon and cheese. It was also different because it came with a packet of red pepper that was powdered instead of in flakes and a packet of powdered oregano.  It tasted great, and it was a nice reminder of home!

Monday, June 23, 2014

The Market, the Potato Selection, (My Sad Lack of a Weekend), and the Scavenger Hunt!


I apologize for not posting for several days! My plans of blogging Thursday night were sidelined until tonight as I got sick for several days.  It was not fun.  But enough on that.

Wednesday

At breakfast on Wednesday, I had the usual coffee and roll, but they also included some yogurt. Without a spoon.  So I ended up half slurping half drinking the yogurt that I’m fairly certain was strawberry flavored, and it tasted great!

Once I got to CIP with Liliam, I headed up to my desk.  Overall, the day was fairly uneventful. The day before, Flavio had begun to teach me how to utilize programming language to format publications and analyze data.  So, in my very basic understanding of these programs, I believe that LaTeX is a computer programming language that is used by most scientific publications for standardizing their format and communication.  So I used LaTeX along with another programming language, simply called R, to format and write different documents that (although I of course would not submit them) technically follow the specifications for various scientific journals.  It was pretty cool, and so far outside of anything I’ve done before! Also, you can use these programming languages to make those complex-looking math problems look nice on the page:

So after a day largely on the computer, I was thrilled when Liliam wanted to stop by the market on the way home!  First we went to a smaller market area that had a roof, but each vendor had their own little stall with walls up.  It was bursting with colors, sounds and smells!

On our way to the market

Liliam buying some herbs

Liliam also bought me some adorably small bananas from a vendor.



Next, we visited the nearby supermarket that was huge! It was a whole three stories with an elevator, packed with just about anything you could want. I didn’t get to wander around much because Liliam was only buying two things, but here a couple pictures:




The bread selection was incredible

Also, my absolute favorite part was that I found some Coke! I hadn't had a cold drink yet, and I was so excited to drink my soon-to-be ice cold Coca Cola.  And to make things better, I got a half a liter (about 16 oz) for just over 60 cents.  I was thrilled!


Thursday

At CIP, Thursday was much more exciting! First, Flavio, Dr. Evelyn Farfan, and I went to the diffused light greenhouse storage room to select 30 seed tubers for each of the six genotypes in our experiment.  It was a long and narrow building, and the interior walkway was flanked with stacks of boxes and crates of potatoes.




We had to match the genotype numbers to the tags on the correct potato crates, which turned out to be a bit more difficult that I expected.  Once we found the corresponding crate, we would gently, so as not to break off the sprouts, inspect and select the best 30 potatoes.  




As I learned, it is best to have a fairly consistent size of tuber for the experiments, while also looking for larger sprouts that had darker or green in color.  After selecting our potatoes, we went over to the greenhouse to help prepare the pots for our experiment. 



After lunch, and another World Cup game, I met with Ximena (pronounced he-men-a) who showed me how to take download data from and program a device that measures heat and relative humidity in the greenhouse.  The device will be placed within a solar UV shield to prevent sunlight from altering the data.



At about 4:00, I left work and walked home because Liliam had to leave without me earlier that afternoon for a family engagement.  I went the same route we take in the morning, so the scenery wasn’t much different, but it was nice to take it in at a more leisurely pace. Once I got home, we ate a dinner of fried eggs and hotdogs (I guess they eat them more like sausages here). 

The nearby agricultural university




After dinner, I began to feel ill, and I spent all of Friday, Saturday, and Sunday home sick.  It wasn’t terribly serious, and I had some medicine prescribed by the doctor at CIP, and I feel much better now! Although I did get up to watch the end of the USA vs. Portugal game, including the heartbreaking, last-second goal by Portugal that tied the game.


Monday

This morning I got to CIP and got Ximena’s help printing the labels for our experiment.  Then Dr. Farfan, Flavio, and I went out to the greenhouse to tie the labels onto stakes that were inserted into the pots we had prepared last week.  And finally it was here: planting time!



First, we carefully checked and re-checked to make sure that the order was correct and all of the potatoes were in the right spot, and then Jorge sprayed a fungicide on them, so we had to wait awhile before re-entering the greenhouse.  Then we carefully matched the seed potato genotypes with their pots and began planting. 



Planting was actually quite simple.  You pick up the potato, push your other hand into the sandy soil mixture and pull back enough to then place the potato in the hole and make sure the sprout is up and then you cover the potato so the sprout is covered in about 1 cm of soil.  One down, 239 more to go!





At lunch, I was able to talk to Lauren, a fellow American (yay!). She studies at the University of Wisconsin-Madison, and has been down to Peru multiple times to work on her ethnobotany (botany paired with the cultural context of the plant) dissertation on oca, a native tuber.  On this trip, Lauren is working on performing genetic analysis as well as helping the nutrition lab with their nutritional analysis of oca. We had a great time talking, and she had some wonderful suggestions on travel destinations within Peru.

After lunch, Flavio explained some of the root analysis that they do, but we were going to test out a new, free program that would hopefully achieve similar results as the current, costly program.  So out we went to find Jorge and begin our scavenger hunt for rogue potato plants that we could dig up.  It was such a nice break, and to make it even better, the sun came out! It was the first time I had truly seen the sun in the eight days I’ve been here, and it was such a pleasant surprise.

Corn seedlings that are used in rotational cropping
So our expedition took us from the weeded parking lot ditches, to the unused fields, to the water canals by the banana trees that separate the fields.  It was quite fun tromping through the fields, and we were fairly successful in finding small plants with decent root systems that we could practice analyzing with the new program.  We placed all the plants in water to prepare them for our efforts tomorrow. 

Little birds that nest in the unused rows (look in the center)


One of the CIP borders

Flavio and Jorge examining one of the plants

Afterwards, I had a few minutes before going home, so I went up and bought myself a coffee from the coffee machine, and sipped it while waiting for Liliam to finish her work.  When we arrived home, it was later than usual so we ate dinner right away, and I had beef! They had purchased some for me and Liliam sliced it thin and cooked it in a pan with salt and pepper.  It was very different than I’m used to, but delicious nonetheless!